Part:BBa_K2314212

UTRrpsT enhanced promoter

UTRrpsT by combining with J23108, to achieve the effect of enhancing J23108. This part contains J23108, UTRrpsT and RBS. UTRrpsT has the ability to enhance the promoter. If you need to enhance the expression of a promoter, you can add UTRrpsT way to achieve. The reason why UTRrpsT is not a separate part is the possibility that the prefix and suffix sequences form a special secondary structure on the 5'UTR. For more details you can view the papers.In short, it can be used as a combination of a promoter and a RBS.

This is the structure of this part.

The enhancement of UTRrpsT may be related to its secondary structure, as shown by the website (http://rna.tbi.univie.ac.at/).

This part derives form part BBa_J23108(https://parts.igem.org/Part:BBa_J23108). 5'UTR will be transcribed and can be used as a regulatory element to adjust the translation process. We chose J23108 and added the 5'UTR sequence (UTRrpsT) thereafter. We found that 6h fluorescence intensity increased by more than 1.5- fold. For more information about our new part, please click here:https://parts.igem.org/Part:BBa_K2314212

UTRrpsT enhances protein expression by structure at the 5’UTR.

FI／Abs600： J23106 vs J23106+UTR

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 56
  
• 1000
  COMPATIBLE WITH RFC[1000]